PUBLICATIONS

Demystifying Functional Parameters for Irreversible Enzyme Inhibitors

David E. Heppner,* Blessing C. Ogboo, Daniel A. Urul, Earl W. May, Erik M. Schaefer, Andrew S. Murkin*, and Matthias Gehringer.

J Med Chem 2024, 67, 14693-14696.

Methylthio-D-ribose-1-Phosphate Isomerase Uses a Novel Mechanism for Aldose–Ketose Isomerization

Subashi Ubayawardhana and Andrew S. Murkin*

ChemRxiv 16 April 2024

Kinetic Characterization of Methylthio-D-ribose-1-phosphate Isomerase

Vamsee M. Veeramachineni, Subashi Ubayawardhana, and Andrew S. Murkin*

Methods in Enzymology 2023, 685, 279-318.

Covalent Adduct Formation in Methylthio-D-ribose-1-Phosphate Isomerase: Reaction Intermediate or Artifact?

Vamsee M. Veeramachineni, Subashi Ubayawardhana, and Andrew S. Murkin*

Biochemistry 2022, 61, 1124-1135.

α-Ketol Rearrangements in Synthetic Organic and Biological Reactions

Scott Benz and Andrew S. Murkin*

Beilstein J Org Chem 2021, 17, 2570-2584.

Inverse Solvent Isotope Effects in Enzyme-Catalyzed Reactions

Patrick L. Fernandez and Andrew S. Murkin*

Molecules 2020, 25, 1933.

New Electrophiles and Strategies for Mechanism-Based and Targeted Covalent Inhibitor Design

Sneha Ray and Andrew S. Murkin*

Biochemistry 2019, 58, 5234-5244.

The Nitro Group as a Masked Electrophile in Covalent Enzyme Inhibition

Sneha Ray, Dale F. Kreitler, Andrew M. Gulick, and Andrew S. Murkin*

ACS Chem Biol 2018, 13, 1470-1473.

Mechanism-based Inactivator of Isocitrate Lyases 1 and 2 from Mycobacterium Tuberculosis

Truc V. Pham, Andrew S. Murkin, Margaret M. Moynihan, Lawrence Harris, Peter C. Tyler, Nishant Shetty, James C. Sacchettini, Hsiao-ling Huang, and Thomas D. Meek*

Proc Nat Acad Sci 2017, 114, 7617-7622.

Ohm’s Law as an Analogy for Enzyme Kinetics

Andrew S. Murkin*

Biochem Mol Biol Educ 2015, 43, 139-141.

The Role of Phosphate in a Multistep Enzymatic Reaction: Comparison of Reactions of the Substrate and Intermediate in PiecesOhm’s Law as an Analogy for Enzyme Kinetics

Svetlana A. Kholodar, C. Leigh Allen, Andrew M. Gulick, and Andrew S. Murkin, A.*

J Am Chem Soc 2015, 137, 2748-2756.

Mechanism and Inhibition of 1-Deoxy-D-xylulose-5-phosphate Reductoisomerase

Andrew S. Murkin,* Kathryn A. Manning, and Svetlana A. Kholodar

Bioorg Chem 2014, 57, 171-185.

Alteration of the Flexible Loop in 1-Deoxy-D-xylulose-5-phosphate Reductoisomerase Boosts Enthalpy-Driven Inhibition by Fosmidomycin

Svetlana A. Kholodar, Gregory Tombline, Juan Liu, Zhesen Tan, C. Leigh Allen, Andrew M. Gulick, and Andrew S. Murkin*

Biochemistry 2014, 53, 3423-3431.

Transition-State-Guided Drug Design for Treatment of Parasitic Neglected Tropical Diseases

Andrew S. Murkin* and Margaret M. Moynihan

Curr Med Chem 2014, 21, 1781-1793.

Cysteine Is the General Base That Serves in Catalysis by Isocitrate Lyase and in Mechanism-Based Inhibition by 3-Nitropropionate

Margaret M. Moynihan and Andrew S. Murkin*

Biochemistry 2014, 53, 178-187.

DXP Reductoisomerase: Reaction of the Substrate in Pieces Reveals a Catalytic Role for the Non-reacting Phosphodianion Group

Svetlana A. Kholodar and Andrew S. Murkin*

Biochemistry 2013, 52, 2302-2308.

Highly Precise Measurement of Kinetic Isotope Effects Using 1H-Detected 2D [13C,1H]-HSQC NMR Spectroscopy

Kathryn A. Manning, Bharathwaj Sathyamoorthy, Alexey Eletsky, Thomas Szyperski, Andrew S. Murkin*

J Am Chem Soc 2012, 134, 20589-20592.

Pre-Steady-State Kinetic Analysis of 1-Deoxy-D-xylulose-5-phosphate Reductoisomerase from Mycobacterium Tuberculosis Reveals Rate-Limiting Product Release by Parallel Pathways

Juan Liu and Andrew S. Murkin*

Biochemistry 2012, 51, 5307-5319.

** joint first author

Cassera, M.B.; Hazleton, K.Z.; Merino, E.F.; Obaldia III, N.; Ho, M.-C.; Murkin, A.S.; DePinto, R.; Gutierrez, J.A.; Almo, S.C.; Evans, G.B.; Babu, Y.S.; Schramm, V.L.  Plasmodium falciparum parasites are killed by a transition state analogue of purine nucleoside phosphorylase in a primate animal model. PLoS ONE 2011, 6, e26916.

Silva, R.G.; Murkin, A.S.; Schramm, V.L. Femtosecond dynamics coupled to chemical barrier crossing in a Born-Oppenheimer enzyme. Proc. Natl. Acad. Sci. USA 2011, 108, 18661-18665.

Silva, R.G.; Hirschi, J.S.; Ghanem, M.; Murkin, A.S.; Schramm, V.L. Arsenate and phosphate as nucleophiles at the transition states of human purine nucleoside phosphorylase. Biochemistry 2011, 50, 2701-2709.

Ghanem, M.**; Murkin, A.S.**; Schramm, V.L. Ribocation Transition State Capture and Rebound in Human Purine Nucleoside Phosphorylase. Chem. Biol. 2009, 16, 971-979.

Bettiol, E.; Samanovic, M.; Murkin, A.S.; Raper, J.; Buckner, F.; Rodriguez, A. Identification of Three Classes of Heteroaromatic Compounds with Activity Against Intracellular Trypanosoma cruzi by Chemical Library Screening. PLoS Negl. Trop. Dis. 2009, 3, e384.

Clinch, K.; Evans, G.B.; Furneaux, R.H.; Kelly, P.M.; Legentil, L.; Murkin, A.S.; Li, L.; Schramm, V.L.; Tyler, P.C.; Woolhouse, A.D. Third Generation Immucillins: Acyclic Immucillins as Inhibitors of Purine Nucleoside Phosphorylase J. Med. Chem. 2009, 52, 1126-1143.

Murkin, A.S.; Clinch, K.; Mason, J.M.; Tyler, P.C.; Schramm, V.L. Immucillins in Custom Catalytic-Site Cavities Bioorg. Med. Chem. Lett. 2008, 18, 5900-5903 (Special issue invitation).

Mason, J.M.; Murkin, A.S.; Li, L.; Schramm, V.L.; Gainsford, G.J.; Skelton, B.W. A b‑Fluoroamine Inhibitor of Purine Nucleoside Phosphorylase J. Med. Chem. 2008, 51, 5880-5884.

Evans, G.B.; Furneaux, R.H.; Greatrex, B.; Murkin, A.S.; Schramm, V.L.; Tyler, P.C. Azetidine Based Transition State Analogue Inhibitors of N-Ribosyl Hydrolases and Phosphorylases J. Med. Chem. 2008, 51, 948-956.

Murkin, A.S.; Tyler, P.C.; Schramm, V.L. Transition State Interactions Revealed in Purine Nucleoside Phosphorylase by Binding Isotope Effects J. Am. Chem. Soc. 2008, 130, 2166-2167.

Rinaldo-Matthis, A.; Murkin, A.S.; Ramagopal, U.; Clinch, K.; Mee, S.P.H.; Evans, G.B.; Tyler, P.C.; Furneaux, R.H.; Almo, S.C.; Schramm, V.L. L-Enantiomers of Transition State Analogue Inhibitors Bound to Human Purine Nucleoside Phosphorylase J. Am. Chem. Soc. 2008, 130, 842-844.

Murkin, A.S.; Birck, M.R.; Rinaldo-Matthis, A.; Shi, W.; Taylor, E.A.; Almo, S.C.; Schramm, V.L. Neighboring Group Participation in the Transition State of Human Purine Nucleoside Phosphorylase Biochemistry 2007, 46, 5038-5049.

Deng, H.; Murkin, A.S.; Schramm, V.L. Phosphate Activation in the Ground State of Purine Nucleoside Phosphorylase J. Am. Chem. Soc. 2006, 128, 7765-7771.

Taylor Ringia, E.A.; Tyler, P.C.; Evans, G.B.; Furneaux, R.H.; Murkin, A.S.; Schramm, V.L. Transition State Analogue Discrimination by Related Purine Nucleoside Phosphorylases J. Am. Chem. Soc. 2006, 128, 7126-7127.

Murkin, A.S.; Chou, W.K.; Wakarchuk, W.W.; Tanner, M.E. Identification and Mechanism of a Bacterial Hydrolyzing UDP-N-Acetylglucosamine 2-Epimerase Biochemistry 2004, 43, 14290-14298.

Vann, W.F.; Daines, D.A.; Murkin, A.S.; Tanner, M.E.; Chaffin, D.O.; Rubens, C.E.; Vionnet, J.; Guerry, P.; Silver, R.P. The NeuC Protein of Escherichia coli K1 Is a Uridine Diphosphate N-Acetylglucosamine 2-Epimerase J. Bacteriol. 2004, 186, 706-712.

Murkin, A.S.; Tanner M. E. Dehydroalanine-Based Inhibition of a Peptide Epimerase from Spider Venom J. Org. Chem. 2002, 67, 8389-8394.